How does the dipstick detect bilirubin, and what factors can affect its accuracy?

How does the dipstick detect bilirubin, and what factors can affect its accuracy?

• A. Bilirubin is detected via the diazo reaction; photo-degradation and light exposure can cause false negatives; test promptly and protect from light.
• B. Bilirubin is detected by an enzymatic color change; high pH can cause false positives.
• C. Bilirubin is measured by a spectrophotometric method; storage temperature does not affect accuracy.
• D. Bilirubin is not detected by dipsticks; only urobilinogen is measured.

Answer: (B)